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Association of hepatitis C virus & hepatitis B virus in chronic liver disease

N Berry1, A Chakravarti, P Kar

  • 1Department of Microbiology, Maulana Azad Medical College, New Delhi.

The Indian Journal of Medical Research
|January 27, 1999
PubMed

Insights

Hepatitis C virus (HCV) is a significant cause of chronic liver disease (CLD), often co-infecting with Hepatitis B virus (HBV). Combining ELISA and RT-PCR offers reliable HCV detection.

Area of Science:

  • Hepatology
  • Virology
  • Clinical Diagnostics

Background:

  • Chronic liver disease (CLD) encompasses conditions like chronic hepatitis, cirrhosis, and hepatocellular carcinoma.
  • Hepatitis C virus (HCV) is a major global health concern contributing to CLD.
  • Understanding the interplay between HCV, Hepatitis B virus (HBV), and CLD is crucial for effective management.

Purpose of the Study:

  • To assess the role of HCV in causing CLD among 55 patients.
  • To determine the prevalence of HCV RNA and its correlation with serological markers.
  • To evaluate the co-infection rate of HBV in HCV-positive CLD patients.

Main Methods:

  • Serum samples from 55 CLD patients were tested for anti-HCV antibodies using ELISA.
  • HCV RNA was detected via reverse transcription polymerase chain reaction (RT-PCR).
  • HBV markers (anti-HBc IgG/IgM, HBsAg) were assessed to evaluate HBV co-infection.

Main Results:

  • HCV infection was detected in 40% (22/55) of CLD patients.
  • A significant proportion (77.3%) of HCV-positive patients showed evidence of HBV infection or past exposure.
  • ELISA and RT-PCR demonstrated comparable sensitivity for HCV detection (P > 0.05).

Conclusions:

  • HCV is a primary cause of CLD, second only to HBV in this patient cohort.
  • HCV and HBV frequently co-infect patients with CLD.
  • Concomitant use of ELISA and RT-PCR is recommended for accurate HCV diagnosis.

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