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Application of authentic peroxynitrite to biological materials
H Ishida1, C Genka, H Nakazawa
1Department of Physiology, School of Medicine, Tokai University, Kanagawa, Japan.
Methods in Enzymology
|January 27, 1999
Abstract:
A method to expose cultured cardiac myocytes to authentic ONOO- by using a constant perfusion system was described. The perfusion apparatus is linked to a microscope equipped with video and fluorescence measurement systems, which enable the evaluation of myocardial contraction, [Ca2+]i, [pH]i, and membrane fluidity. Special care is needed to adjust the pH of the solution appropriately and to obtain a constant concentration of ONOO-.