Genetic analysis of the cap5 locus of Staphylococcus aureus

Staphylococcus aureus expresses at least eight distinct serotypes of capsular polysaccharide (CP). Gene clusters involved in the expression of serotypes 1, 5 and 8 have been cloned and sequenced. In this report we describe the isolation and analysis of serotype 5 capsular polysaccharide-defective mutants. A naturally occurring cap mutation in the laboratory strains 8325-4 and RN4220 was mapped to the cap5E gene by genetic complementation. The cap5H-K genes were shown to be responsible for CP5 serotype specificity by transduction and complementation.