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Related Experiment Video

Updated: Jul 2, 2026

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
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Minimization of CD34+ cell enumeration variability using the ProCOUNT standardized methodology

I McNiece1, B Kern, K Zilm

  • 1Department of Developmental Hematology, Amgen Incorporated, Thousand Oaks, CA 91320, USA.

Journal of Hematotherapy
|January 27, 1999
PubMed
Summary

The ProCOUNT system automates CD34+ cell analysis, reducing operator variability and improving consistency in cell enumeration for stem cell transplantation. This automated method offers a reliable alternative to standard procedures.

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Area of Science:

  • Hematology
  • Immunology
  • Biotechnology

Background:

  • CD34+ cell dose is a key predictor of engraftment after stem cell transplantation.
  • Standard CD34+ cell analysis methods exhibit operator and technical variability.
  • Automated systems are needed to improve the reliability and standardization of CD34+ cell enumeration.

Purpose of the Study:

  • To evaluate the performance of the Becton Dickinson ProCOUNT cell enumeration kit.
  • To compare the ProCOUNT system with a standard CD34+ cell analysis methodology.
  • To assess the impact of ProCOUNT on inter-operator variability and consistency.

Main Methods:

  • The ProCOUNT system was evaluated against a standard CD34+ cell analysis (Milan approach).
  • Analyses were performed on leukapheresis products from patients and donors mobilized with chemotherapy plus G-CSF or G-CSF alone.
  • CD34+ cell-selected products with purities >75% were also analyzed.
  • The standard method identified CD45+, low side scatter, and CD34+ cells.

Main Results:

  • High correlation coefficients were observed between ProCOUNT and standard methods for various sample types (r=0.83 to 0.98).
  • ProCOUNT significantly reduced inter-operator variability (r=0.99) compared to the standard method (r=0.77).
  • ProCOUNT enables absolute CD34+ cell concentration determination, bypassing WBC count errors.

Conclusions:

  • The ProCOUNT system demonstrates high consistency with existing CD34+ cell analysis methodologies.
  • ProCOUNT significantly minimizes variability between different operators, enhancing standardization.
  • This automated system offers a reliable and potentially standardized approach for CD34+ cell enumeration in clinical laboratories.