Analysis of gentamicin by liquid chromatography with pulsed electrochemical detection
Related Experiment Video
Updated: Oct 26, 2026

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
Published on: March 3, 2023
E Adams1, W Roelants, R De Paepe
1Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Belgium.
Abstract:
The analysis of gentamicin by liquid chromatography using a column packed with poly(styrene-divinylbenzene) and pulsed electrochemical detection on a gold electrode is described. The mobile phase consists of an aqueous solution containing sodium sulfate, tetrahydrofuran, sodium 1-octanesulfonate and a phosphate buffer of pH 3.0. In contradistinction to methods previously published, this method not only allows a better separation of gentamicins C1, C1a, C2, C2a and C2b, but also the separation of several other, minor components, most of which were not identified. The effects of the different chromatographic parameters on the separation were also investigated. A number of commercial samples was analysed using this method, allowing sensitive detection of gentamicin without derivatization, and the results were compared with the results obtained with the European Pharmacopoeia method, prescribing pre-column derivatization.
Related Concept Videos
High-Performance Liquid Chromatography: Types of Detectors
Determination of Multiple Dosing Parameters: Steady-State, Minimum and Maximum Concentrations
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary Electrophoresis: Instrumentation
Gas Chromatography–Mass Spectrometry (GC–MS)
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating...

