Related Experiment Videos
Novel insights into structure and function of MRP8 (S100A8) and MRP14 (S100A9)
1Institut für Experimentelle Dermatologie, Münster, Germany. kerkhoc@uni-muenster.de
Abstract:
The two migration inhibitory factor- (MIF)-related protein-8 (MRP8; S100A8) and MRP14 (S100A9) are two calcium-binding proteins of the S100 family. These proteins are expressed during myeloid differentiation, are abundant in granulocytes and monocytes, and form a heterodimeric complex in a Ca2+-dependent manner. Phagocytes expressing MRP8 and MRP14 belong to the early infiltrating cells and dominate acute inflammatory lesions. In addition, elevated serum levels of MRP8 and MRP14 have been found in patients suffering from a number of inflammatory disorders including cystic fibrosis, rheumatoid arthritis, and chronic bronchitis, suggesting conceivable extracellular roles for these proteins. Although a number of possible functions for MRP8/14 have been proposed, the biological function still remains unclear. This review addresses recent developments regarding the MRP14-mediated promotion of leukocyte-endothelial cell-interactions and the characterization of MRP8/14 heterodimers as a fatty acid binding protein complex. In view of the current knowledge, the authors will hypothesize that MRP8 and MRP14 play an important role in leukocyte trafficking, but do not affect neutrophil effector functions.
Insights
Migration inhibitory factor-related proteins 8 (MRP8) and 14 (MRP14) are key in inflammatory lesions. This review explores their role in leukocyte trafficking, not neutrophil functions.
Area of Science:
- Immunology
- Molecular Biology
- Biochemistry
Background:
- MRP8 (S100A8) and MRP14 (S100A9) are calcium-binding proteins abundant in myeloid cells.
- These proteins form a heterodimer crucial in early inflammatory cell infiltration.
- Elevated serum levels in inflammatory diseases suggest extracellular functions.
Purpose of the Study:
- To review recent findings on MRP14's role in leukocyte-endothelial cell interactions.
- To characterize MRP8/14 heterodimers as a fatty acid binding complex.
- To hypothesize the function of MRP8 and MRP14 in leukocyte trafficking and neutrophil effector functions.
Main Methods:
- Literature review of recent developments in MRP8/14 research.
- Analysis of studies on leukocyte-endothelial cell interactions.
- Characterization of MRP8/14 heterodimer properties.
Main Results:
- MRP14 mediates leukocyte-endothelial cell interactions.
- MRP8/14 heterodimers function as a fatty acid binding complex.
- Evidence suggests MRP8/14 are involved in leukocyte trafficking.
Conclusions:
- MRP8 and MRP14 play a significant role in leukocyte trafficking.
- These proteins do not appear to affect neutrophil effector functions.
- Further research is needed to fully elucidate the biological functions of MRP8/14.