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Transfer of sulfur to biotin from biotin synthase (BioB protein)
K J Gibson1, D A Pelletier, I M Turner
1E.I. DuPont de Nemours, Inc., Wilmington, Delaware, 19880-0328, USA.
Biochemical and Biophysical Research Communications
|January 28, 1999
Abstract:
In biotin synthase reactions carried out in vitro, we observed efficient transfer of 35S to biotin from partially purified E. coli biotin synthase (product of the bioB gene) labelled by biosynthetic incorporation of [35S]-cysteine. Mass spectrometry was consistent with covalent alteration of the protein in the assay. These results suggest that BioB protein is a reagent, not a catalyst.