Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CCG repeats in cDNAs from human brain

J J Kleiderlein1, P E Nisson, J Jessee

  • 1Department of Psychiatry, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

Human Genetics
|January 28, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plasma 8-hydroxy-2'-deoxyguanosine Levels in Huntington Disease and Healthy Controls Treated with Coenzyme Q10.

Journal of Huntington's disease·2014
Same author

Protocol for an HTA report: Does therapeutic writing help people with long-term conditions? Systematic review, realist synthesis and economic modelling.

BMJ open·2014
Same author

A method of teaching and evaluating psychiatric case formulation.

Academic psychiatry : the journal of the American Association of Directors of Psychiatric Residency Training and the Association for Academic Psychiatry·2014
Same author

Solvothermal annealing of block copolymer thin films.

Nano letters·2013
Same author

Magnetic entropy change plateau in a geometrically frustrated layered system: FeCrAs-like iron-pnictide structure as a magnetocaloric prototype.

Journal of physics. Condensed matter : an Institute of Physics journal·2013
Same author

Self-assembled incorporation of modulated block copolymer nanostructures in phase-change memory for switching power reduction.

ACS nano·2013

Researchers identified new human brain cDNAs with unstable DNA repeats, potential genetic markers for neuropsychiatric disorders like schizophrenia and autism. These findings aid the search for disease-associated expansion mutations.

Area of Science:

  • Genetics
  • Neuroscience
  • Molecular Biology

Background:

  • Expansion mutations in unstable DNA repeats are implicated in neuropsychiatric disorders such as bipolar disorder, schizophrenia, autism, and panic disorder.
  • Identifying novel genes associated with these repeats is crucial for understanding genetic susceptibility.

Purpose of the Study:

  • To discover new candidate genes for neuropsychiatric disorders by identifying cDNAs with specific DNA repeat sequences in the human brain.
  • To characterize these novel cDNAs for their chromosomal location, repeat length polymorphism, and functional similarity to known genes.

Main Methods:

  • Probing human brain cDNA libraries at high stringency for clones containing CCG, CGC, GCC, CGG, GCG, and GGC repeats.
  • Characterizing identified cDNAs for chromosomal locus, repeat length polymorphism, and homology to known genes.

Related Experiment Videos

  • Comparing novel repeats with existing human genes containing CCG triplets in GenBank.

    • Main Results:

    • 18 cDNAs with previously uncharacterized repeats were identified and mapped to various chromosomal loci.
    • Fifty percent of the identified repeats exhibited length polymorphism.
    • Newly cloned cDNAs include human neurexin-1B, BCNG-1 (a brain-specific ion channel), a polymorphic repeat in the G-protein beta2 subunit, and human proline-rich protein 7.

    Conclusions:

    • The identified cDNAs represent novel candidate genes for neuropsychiatric disorders.
    • The characterization of these repeats and their polymorphisms provides valuable resources for genetic studies.
    • This research facilitates the ongoing search for expansion mutations linked to central nervous system diseases.