Microwave disinfection of denture base materials colonized with Candida albicans

Infection of denture materials with Candida albicans is common and contributes to denture stomatitis.

This 3-phase investigation examined: (1) the efficacy of microwave irradiation against C albicans colonized on 3 soft denture liners and 1 heat-polymerized denture base resin, and (2) the effect of this irradiation on the hardness of the materials tested.

In phase 1, an experimental protocol was developed. Sterilized specimens from 2 denture base soft liners and 1 heat-polymerized acrylic resin denture base material (n = 45 each) were inoculated with C albicans. Two thirds of the specimens were irradiated in a 60 Hz microwave oven for 5 minutes (dry). C albicans growth was then assessed with streaked blood agar plates and thioglycollate broth. One third of the specimens were not irradiated and served as controls. Pretest and posttest Shore A hardness values were obtained and compared. For phase 2, 15 specimens from each material group were subjected to irradiation (while immersed in water) for 5 minutes; and, 15 from each material were subjected to 10- and 15-minute irradiation (dry), with subsequent sterility and change in hardness assessments completed as described in phase 1. In phase 3, 15 specimens from each material group were subjected to repeated 5-minute irradiation cycles (while immersed in water), and changes in hardness were examined.

Only the 5-minute irradiated specimens immersed in water were effectively sterilized, as verified by the thioglycollate assay. The effect of repeated 5-minute irradiation cycles resulted in a significant change in hardness of the PermaSoft specimens.

Five-minute irradiation, while immersed in water, killed all C albicans present on the materials tested; and, repeated 5-minute irradiation significantly affected the hardness of only the PermaSoft material.