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Coronary Progenitor Cells and Soluble Biomarkers in Cardiovascular Prognosis after Coronary Angioplasty
Published on: January 28, 2020
Association between the degree of platelet-derived growth factor-A chain mRNA expression and coronary
1Department of Cardiovascular Surgery, Heart Institute of Japan, Tokyo Women's Medical University, Japan.
Insights
Platelet-derived growth factor A (PDGF-A) is crucial in graft coronary arteriosclerosis (GCA). Higher PDGF-A expression in cardiac allografts strongly correlates with increased intimal thickening and diseased vessels in GCA.
Area of Science:
- Cardiovascular Research
- Immunology
- Regenerative Medicine
Background:
- Graft coronary arteriosclerosis (GCA) is a significant complication following heart transplantation.
- Smooth muscle cell proliferation in the intima and media are key mechanisms in GCA development.
- The precise role of platelet-derived growth factor (PDGF) in GCA pathogenesis remains unclear.
Purpose of the Study:
- To investigate the correlation between the extent of GCA and platelet-derived growth factor A (PDGF-A) chain expression in cardiac grafts.
- To elucidate the role of PDGF-A in the development of GCA.
Main Methods:
- Heterotopic heart transplantation was performed in 21 rats.
- Rats received immunosuppressive treatments: cyclosporine A, 15-deoxyspergualin, or Multiglycosidorum tripterygii (MT).
- Histological evaluation of GCA and Northern blot analysis for PDGF-A chain mRNA expression were conducted 60 days post-transplantation.
Main Results:
- Varying degrees of GCA were observed across the transplanted hearts.
- A significant positive correlation was found between PDGF-A mRNA expression and the grade of arterial intimal thickening (Spearman's r = 0.76, P < 0.005).
- PDGF-A mRNA expression also showed a strong correlation with the incidence of diseased vessels (r = 0.82, P < 0.001).
Conclusions:
- PDGF-A chain mRNA expression in cardiac allografts is significantly associated with the extent of GCA.
- These findings indicate that PDGF-A plays a critical role in the development and progression of GCA.
- Targeting PDGF-A may offer a therapeutic strategy for preventing or treating GCA.
Abstract:
Although intimal and medial proliferation of smooth muscle cells is recognized as one of the key mechanisms in the development of graft coronary arteriosclerosis, the role of platelet-derived growth factor (PDGF) in this process is still uncertain, because of the undetermined pathogenesis of graft coronary arteriosclerosis (GCA). In the present study, the correlation between the extent of GCA and the degree of PDGF-A chain expression in cardiac grafts was investigated in 21 rats with GCA of varying extent. Lewis rats underwent heterotopic heart transplantation from Wistar King donors and were treated with cyclosporine A (10 mg/kg/day) (n = 7), 15-deoxyspergualin (5 mg/kg/day) (n = 7), or Multiglycosidorum tripterygii (MT) (30 mg/kg/day) (n = 7). Histological evaluations of coronary arteriosclerosis, as well as Northern blot analysis of graft PDGF-A chain expression were made, 60 days after transplantation. Graft coronary arteriosclerosis of varying extent was observed among the 21 transplanted hearts. Significant correlations were found between the PDGF-A chain mRNA expression of cardiac allograft and the grade of arterial intimal thickening (Spearman's r = 0.76, P < 0.005) as well as the incidence of diseased vessels (r = 0.82, P < 0.001). The PDGF-A chain mRNA expression of the cardiac allograft is associated with the extent of GCA, indicating that PDGF-A plays an important role in the development of GCA.
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