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A transient assay for evaluating promoters in wheat endosperm tissue
1C.S.I.R.O., Division of Plant Industry, A.C.T., Australia. b.clarke@pi.csiro.au
Abstract:
A transient assay was developed for the evaluation of promoter sequences in wheat endosperm tissue. A deletion series from an omega-secalin gene promoter, located on chromosome 1RS.1DL of specific wheat lines, were translationally fused to a uidA reporter gene. These promoters were evaluated for expression in wheat endosperm tissue after integration of the DNA into the cell using microprojectile bombardment. The results were compared with those obtained using other transient assay systems.
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