Evaluation of immunomagnetic separation for recovery of infectious Cryptosporidium parvum oocysts from environmental samples
Related Experiment Video
Updated: Oct 7, 2026
Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient
Published on: November 4, 2009
P A Rochelle1, R De Leon, A Johnson
1Water Quality Laboratory, Metropolitan Water District of Southern California, La Verne, California 91750-3399, USA. prochelle@mwd.dst.ca.us
Abstract:
Two commercial immunomagnetic separation (IMS) kits for Cryptosporidium were compared for recovery of oocysts from environmental samples. Oocyst recovery efficiencies with the Dynal and Crypto-Scan kits ranged from 62 to 100% and 34 to 74%, respectively, for seeded environmental water concentrates (turbidity of 210 to 11,480 nephelometric turbidity units). Recovery efficiencies were dependent on the mechanism of agitation during the magnetic capture procedure. An assay combining in vitro cell culture and reverse transcriptase PCR demonstrated that oocysts recovered by IMS retained their infectivity.
More Related Videos
12:11A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
Published on: July 9, 2012
05:31Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection
Published on: September 14, 2019