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Insulin modulates cellular proliferation in developing human jejunum and colon

D Ménard1, L Corriveau, J F Beaulieu

  • 1Groupe de Recherche en Biologie du Développement, Département d'Anatomie et de Biologie Cellulaire, Faculté de Médecine, Université de Sherbrooke, Qué., Canada. dmenard@courrier.usherb.ca

Biology of the Neonate
|February 2, 1999
PubMed
Summary

Insulin receptors are present in the developing human gut. Insulin stimulates the proliferation of epithelial cells in the fetal jejunum and colon, but does not affect enzyme activity.

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Area of Science:

  • Gastroenterology
  • Developmental Biology
  • Endocrinology

Background:

  • Insulin's role in rodent intestinal development is known.
  • Its function in human gut development requires further investigation.

Purpose of the Study:

  • To investigate the presence of insulin receptors (IR) in the human fetal gut.
  • To determine the effect of insulin on cell proliferation and enzyme activity in the developing human jejunum and colon.

Main Methods:

  • Immunofluorescent localization of insulin receptors (IR) in fetal jejunum and colon (14-19 weeks).
  • Cultured explants were treated with insulin to assess [3H]-thymidine incorporation and enzyme activities (lactase, alkaline phosphatase).

Main Results:

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  • Insulin receptors (IR) were detected in the basolateral portion of enterocytes and colonocytes.
  • Insulin significantly increased [3H]-thymidine incorporation and epithelial labeling indices in both jejunum and colon explants.
  • Insulin did not affect lactase or alkaline phosphatase activities.

    • Conclusions:

    • This study confirms the early presence of insulin receptors (IR) in the developing human gut.
    • Insulin stimulates epithelial cell proliferation in the fetal jejunum and colon.
    • Insulin does not influence brush border hydrolase activities in these developing tissues.