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Updated: Aug 5, 2026

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
Published on: December 10, 2012
Differential destabilization of repetitive sequence hybrids in fluorescence in situ hybridization
J C Hozier1, J M Scalzi, A C Clase
1Applied Genetics Laboratories, Inc., Melbourne, FL, (USA). jh@appliedgenetics.com
Abstract:
A method for painting a chromosome or chromosome region by fluorescence in situ hybridization (FISH) without blocking DNA is described. Both unique sequence and repetitive sequence components of a fluorescently labeled probe are hybridized under low-stringency conditions, but the chromosomes are washed in such a manner that repetitive sequences are differentially removed, while region-specific unique sequence fragments remain bound to the target chromosomes. We refer to this differential retention and removal of probe components as differential stability FISH.
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