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Receptor mobility and its cooperative restriction by ligands
Immunological Communications
|January 1, 1976
Summary
Ligand binding, like with Concanavalin A, modifies lymphoid cell membranes, restricting receptor mobility. This process involves receptor recruitment and is influenced by submembrane structures and specific drugs.
Area of Science:
- Immunology
- Cell Biology
- Biophysics
Background:
- Membrane fluidity and receptor mobility are crucial for lymphoid cell function.
- Ligand-induced restriction of receptor mobility is a recently observed phenomenon.
Purpose of the Study:
- To investigate the cooperative binding of Concanavalin A to lymphoid cells.
- To characterize the micro-redistribution of receptors and its modulation by ligands and drugs.
Main Methods:
- Quantitative measurement of active sites on cell-bound lectin using horse-radish peroxidase fixation.
- Morphological techniques to study receptor mobility and micro-redistribution at 4°C.
Main Results:
- Concanavalin A binding induces membrane modification and receptor recruitment, demonstrating cooperative binding.
- A post-binding event, micro-redistribution, occurs at low temperatures and is cooperatively restricted.
- Colchicine and cytochalasin B modulate these phenomena, suggesting dependence on submembrane structures.
Conclusions:
- Receptor density and distribution are regulated by a temperature-stable multimeric submembrane structure.
- Ligand-induced receptor micro-redistribution is a cooperative process modulated by cellular components.