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Updated: Aug 15, 2026

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
Published on: March 3, 2015
Regulation of levels of 5-HT2A receptor mRNA
1Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA.
Abstract:
We have been able to identify two separate pathways present in P11 cells that can increase the levels and stability of 5-HT2A receptor mRNA. One pathway is activated following exposure of cells to serotonin and is dependent upon activation of a PKC isoform that is susceptible to downregulation by a 24-h pretreatment with PMA; another pathway is activated following treatment of cells with a calcium ionophore. This pathway is not dependent on activation of PKC isoforms that can be downregulated by a 24-h treatment with phorbol ester. Such heterologous regulation of levels of 5-HT2A receptor mRNA may be important in understanding in vivo events where multiple factors contribute to the modulation of levels of cell surface receptors.
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