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Related Experiment Videos

Serotonin transporter function in vivo: assessment by chronoamperometry

A Frazer1, L C Daws

  • 1Department of Pharmacology, University of Texas Health Science Center at San Antonio 78250, USA. frazer@uthscsa.edu

Annals of the New York Academy of Sciences
|February 3, 1999
PubMed
Summary

Selective serotonin reuptake inhibitors (SSRIs) and norepinephrine transporters (NETs) influence serotonin clearance in the hippocampus. Autoreceptor activation may enhance serotonin transporter kinetics.

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Area of Science:

  • Neuroscience
  • Pharmacology

Background:

  • Serotonin (5-HT) signaling is crucial for hippocampal function.
  • The mechanisms regulating serotonin clearance in specific hippocampal subregions are not fully understood.

Purpose of the Study:

  • To investigate the roles of serotonin and norepinephrine transporters in the clearance of exogenous serotonin in the rat dorsal hippocampus.
  • To explore the influence of serotonin terminal autoreceptors on serotonin clearance.

Main Methods:

  • In vivo chronoamperometry was used to measure serotonin clearance.
  • Selective serotonin reuptake inhibitors (fluvoxamine, citalopram) and NE uptake inhibitors (desipramine, protriptyline) were applied locally.
  • Neurotoxic agents (5,7-dihydroxytryptamine, 6-hydroxydopamine) were used to deplete specific neurotransmitter systems.

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  • Cyanopindolol, a serotonin terminal autoreceptor antagonist, was administered.

    • Main Results:

    • SSRIs prolonged serotonin clearance in the dentate gyrus and CA3 region.
    • NE uptake inhibitors prolonged serotonin clearance in the dentate gyrus but not CA3.
    • These transporter effects were dependent on intact serotonergic and noradrenergic systems, respectively.
    • Autoreceptor antagonism also prolonged serotonin clearance in the CA3 region.

    Conclusions:

    • Both serotonin transporter (SERT) and norepinephrine transporter (NET) contribute to serotonin clearance in the dentate gyrus.
    • Serotonin terminal autoreceptor activation appears to enhance the kinetics of serotonin uptake via SERT, particularly in the CA3 region.