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Related Experiment Videos

Chromosomal damage rate, aging, and diet

M Fenech1

  • 1CSIRO Division of Human Nutrition, Adelaide SA, Australia. michael.fenech@dhn.csiro.au

Annals of the New York Academy of Sciences
|February 3, 1999
PubMed
Summary
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Aging increases chromosomal damage, but diet and vitamins B12 and folic acid may offer protection. Moderate red wine consumption also showed protective effects against DNA damage.

Area of Science:

  • Genetics
  • Nutritional Science
  • Gerontology

Background:

  • Chromosomal damage, including micronucleus (MN) formation, increases with age.
  • Aging explains at least 25% of the variation in chromosomal damage rate.
  • Dietary factors and vitamin status are potential modulators of chromosomal damage.

Purpose of the Study:

  • To investigate the relationship between MN frequency and diet, including vitamin B12, folic acid, C, and E status.
  • To explore the impact of aging on chromosomal damage rates.
  • To assess the protective effects of wine consumption against DNA damage.

Main Methods:

  • Cytokinesis-block micronucleus technique for measuring chromosomal breakage and loss.
  • Cross-sectional and placebo-controlled intervention studies.

Related Experiment Videos

  • Analysis of MN frequency in relation to diet, vitamin status, and homocysteine levels.
  • Ex vivo testing of wine's effect on hydrogen peroxide-induced MN frequency.

    • Main Results:

    • MN frequency correlated negatively with folic acid and vitamin B12 levels.
    • Optimal MN frequency observed with plasma B12 > 300 pmol/L and homocysteine < 7.5 mumol/L.
    • High-dose vitamin E or folic acid interventions did not significantly reduce MN frequency.
    • Red wine consumption showed significant ex vivo inhibition of DNA damage.

    Conclusions:

    • Chromosomal damage is influenced by aging and can be modulated by dietary factors.
    • Vitamin B12 and folic acid status are important for minimizing MN frequency.
    • Moderate red wine consumption may offer protection against DNA damage.