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Does glutaraldehyde induce calcification of bioprosthetic tissues?
A Vincentelli1, C Latrémouille, R Zegdi
1Department of Cardiovascular Surgery, Cardiologic Hospital, Lille, France. avincent@club-internet.fr
The Annals of Thoracic Surgery
|February 4, 1999
Summary
Tissue origin, not glutaraldehyde treatment duration, significantly impacts calcification in implanted pericardial samples. Autologous tissues showed lower calcification than heterologous tissues, regardless of glutaraldehyde exposure time.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Biocompatibility Studies
Background:
- Glutaraldehyde is implicated in the calcification of treated tissues post-implantation.
- The role of tissue origin (autologous vs. heterologous) in this calcification process requires further investigation.
Purpose of the Study:
- To determine if tissue origin (autologous vs. heterologous) plays a more significant role than glutaraldehyde treatment in tissue calcification.
- To assess the impact of varying glutaraldehyde treatment durations on calcification.
Main Methods:
- Sheep pericardial and human pericardial samples were implanted subcutaneously in sheep.
- Samples were treated with 0.6% glutaraldehyde for different durations (5 min to 7 days) or not treated.
- Calcium content was measured after 3 months post-implantation.
Main Results:
- Heterologous tissues exhibited significantly higher calcium content compared to autologous tissues.
- The duration of glutaraldehyde treatment did not influence the calcium content in either tissue type.
- Autologous tissues demonstrated a consistently low level of calcification.
Conclusions:
- Glutaraldehyde treatment duration is not a significant factor in the calcification of implanted pericardial tissue.
- Tissue origin is a critical determinant of calcification, with autologous tissues showing reduced calcification.
- Autologous tissues represent a potentially more calcification-resistant option compared to heterologous tissues.