Related Experiment Videos
Down-regulation of beta1,4-galactosyltransferase gene expression by cell-cycle suppressor gene p16
1Key Laboratory of Glycoconjugate, Ministry of Public Health, P.R. of China, and Gene Research Center, Shanghai Medical University, Shanghai 200032, PR China.
Abstract:
Beta1,4-Galactosyltranferase (beta1,4GT, EC 2.4.1.38) is one of the key enzymes controlling the biosynthesis of complex-type oligosaccharides, and is also one of the best-studied glycosyltransferases. To study the molecular mechanisms involved in the regulation of beta1,4GT gene expression, we transfected cell-cycle suppressor gene p16 into A549 cell line (in which p16 is deleted), measured beta1,4GT gene expression by Northern blot hybridization, and evaluated its activity. It was found that p16 could down-regulate beta1,4GT gene expression and its activity. However, p16 decreased cell surface beta1,4GT activity more than total activity. beta1,4GT mRNA stability was also assayed. It was found that p16 could not influence beta1,4GT mRNA stability.