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Updated: Oct 25, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
Published on: September 19, 2018
A simple method for the determination of isoelectric points of ampholytes with closely spaced pKa values using
1Chemistry Department, Texas A&M University, College Station 77842-3012, USA.
Abstract:
A simple method, based on a modified version of pressure-mediated capillary electrophoresis (PreMCE) has been developed for the determination of the isoelectric points of ampholytes which have closely spaced pKa values. This new pI-determination PreMCE method (i) can be easily executed on most commercial capillary electrophoresis instruments; (ii) it can use small, impure samples, (iii) unlike isoelectric focusing methods in natural pH gradients, it does not require a linear pH gradient, and (iv) it eliminates the pI errors that are due to chromatographic retention on the walls of the separation chamber.
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