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CD20 is physically and functionally coupled to MHC class II and CD40 on human B cell lines
C Léveillé1, R AL-Daccak, W Mourad
1Centre de Recherche en Rhumatologie et Immunologie, CHUL, St-Foy, Québec, Canada.
European Journal of Immunology
|February 5, 1999
Summary
Monoclonal antibody R21 blocks B cell adhesion by targeting the CD20 molecule, revealing its physical association with MHC class II and CD40, suggesting a modulatory role in B cell function.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Engagement of MHC class II and CD40 on B cells initiates signaling pathways that activate adhesion receptors, leading to cell-cell adhesion.
- This adhesion can be mediated by both LFA-1-dependent and LFA-1-independent mechanisms.
Purpose of the Study:
- To investigate the role of the CD20 molecule in B cell adhesion.
- To determine the relationship between CD20, MHC class II, and CD40 in B cell interactions.
Main Methods:
- Production of a murine monoclonal antibody (mAb R21) against a B cell line.
- Assessing the effect of mAb R21 on MHC class II- and CD40-induced B cell adhesion.
- Biochemical characterization to identify the target of mAb R21 and its molecular associations.
Main Results:
- mAb R21 effectively blocked LFA-1-independent homotypic adhesion induced by MHC class II and CD40.
- mAb R21 triggered LFA-1-dependent cell-cell adhesion on its own.
- CD20 was identified as the target of mAb R21 and is physically associated with MHC class II and CD40 on B cells.
Conclusions:
- CD20 is physically coupled to MHC class II and CD40 molecules on B cells.
- CD20 likely plays a modulatory role in the functions of MHC class II and CD40.
- These findings provide new insights into B cell adhesion and signaling pathways.