Related Experiment Videos
Single-channel recording of a store-operated Ca2+ channel in Jurkat T lymphocytes
1Department of Animal Physiology, Institute of Zoology, University of Salzburg, A-5020 Salzburg, Austria. Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA.
Abstract:
In T lymphocytes, a store-operated calcium ion (Ca2+) entry mechanism termed the calcium release-activated Ca2+ channel (CRAC channel) underlies the sustained or oscillatory intracellular calcium concentration signal required for interleukin-2 gene expression and cell proliferation. The use of sodium ions as a current carrier enabled single-channel recordings of CRAC channels during activation, inactivation, and blockade of current in the presence of divalent cations. A large conductance of 36 to 40 picosiemens indicates that 100 to 400 CRAC channels are present in T lymphocytes.