Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A direct repeat sequence at the Rasgrf1 locus and imprinted expression

R S Pearsall1, C Plass, M A Romano

  • 1Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, New York, 14263-0001, USA.

Genomics
|February 6, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrative analysis of RNA expression signatures and recurrent genomic alterations before treatment: link to menopausal status, short-term endocrine therapy response and disease-free survival in luminal breast cancer.

ESMO open·2025
Same author

Knock-down of oncohistone H3F3A-G34W counteracts the neoplastic phenotype of giant cell tumor of bone derived stromal cells.

Cancer letters·2019
Same author

Genome-wide screen for differentially methylated long noncoding RNAs identifies Esrp2 and lncRNA Esrp2-as regulated by enhancer DNA methylation with prognostic relevance for human breast cancer.

Oncogene·2017
Same author

TET-mediated hydroxymethylcytosine at the Pparγ locus is required for initiation of adipogenic differentiation.

International journal of obesity (2005)·2017
Same author

Stability of the human sperm DNA methylome to folic acid fortification and short-term supplementation.

Human reproduction (Oxford, England)·2016
Same author

IL-10 production by CLL cells is enhanced in the anergic IGHV mutated subset and associates with reduced DNA methylation of the IL10 locus.

Leukemia·2016
Same journal

Integrating transcriptomics and metabolomics reveals the molecular landscape of sperm maturation driven by regional differentiation in the epididymis of Guizhou-Guiqian semi-fine wool sheep.

Genomics·2026
Same journal

Impact of genotype on histopathology and clinical characters in a Chinese cohort with obstructive hypertrophic cardiomyopathy.

Genomics·2026
Same journal

A novel reusable transcriptome-wide association study workflow used to map key genes linked to important cattle traits.

Genomics·2026
Same journal

The large mitochondrial genome of Syndiclis anlungensis (Lauraceae): Genome structure, comparative analysis, and phylogenetic relationships with other Syndiclis species.

Genomics·2026
Same journal

DeepGEP: Deep learning for gene expression prediction from multi-omics in mammals.

Genomics·2026
Same journal

Molecular features of external Auditory Canal cholesteatoma by microbial metagenomic sequencing.

Genomics·2026
See all related articles

Genomic imprinting involves parental-specific gene expression. Researchers found that direct repeat elements are associated with paternal-specific methylation and monoallelic expression of the Rasgrf1 gene in rodents, suggesting their role in imprinting.

Area of Science:

  • Epigenetics
  • Genomics
  • Molecular Biology

Background:

  • Genomic imprinting regulates parental-specific gene expression.
  • CpG methylation is a candidate epigenetic mark, but its establishment and maintenance are poorly understood.
  • The Rasgrf1 gene on mouse chromosome 9 exhibits paternal-specific expression and methylation.

Purpose of the Study:

  • To investigate the role of direct repeat elements in genomic imprinting.
  • To determine if similar repeat structures and methylation patterns exist in other rodent species.
  • To correlate repeat structures with Rasgrf1 gene expression patterns.

Main Methods:

  • Comparative genomic analysis across rodent species (Mus, Rattus, Peromyscus).
  • Southern Blot analysis to detect repeat structures.

Related Experiment Videos

  • Analysis of Rasgrf1 gene expression in neonatal brain tissue.

    • Main Results:

    • Two rodent species (Mus and Rattus) possess direct repeat structures near a paternally methylated region upstream of Rasgrf1.
    • These species exhibit paternal-specific monoallelic expression of Rasgrf1 in neonatal brain.
    • A divergent species (Peromyscus) lacking similar repeat structures shows biallelic Rasgrf1 expression.

    Conclusions:

    • Direct repeat elements are conserved in association with imprinted genes in closely related rodent species.
    • These repeat structures likely play a crucial role in establishing and maintaining genomic imprinting.
    • The presence or absence of repeat elements correlates with monoallelic or biallelic gene expression.