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O-(fluoresceinylmethyl)hydroxylamine (OFMHA): a fluorescent reagent for detection of damaged nucleic acids
M Adamczyk1, P G Mattingly, J A Moore
1Department of Chemistry, Abbott Laboratories, Abbott Park, IL 60064-3500, USA.
Bioorganic & Medicinal Chemistry Letters
|February 6, 1999
Abstract:
5- and 6-O-(Fluoresceinylmethyl)hydroxylamine (OFMHA, 5a, b) were prepared from the corresponding bis-pivaloyl-protected hydroxymethylfluoresceins (1a, b) in 50-70% yield. The hydroxylamine derivatives reacted smoothly with the abasic sites present in acid/heat stressed calf thymus DNA.