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Related Experiment Videos

Temperate coliphage HK022. Clear plaque mutants and preliminary vegetative map

T S Dhillon, E K Dhillon

    Japanese Journal of Microbiology
    |October 1, 1976
    PubMed
    Summary

    Mutagenesis of phage HK022 identified clear plaque mutants, revealing two complementation groups, cI and cII. Temperature-sensitive mutants were characterized, aiding in the construction of a vegetative phage map.

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    Area of Science:

    • Bacteriophage genetics
    • Molecular biology
    • Microbial genetics

    Background:

    • Phage HK022 mutagenesis was performed to isolate clear plaque mutants.
    • Clear plaque mutants are essential for studying phage genetics and life cycles.

    Purpose of the Study:

    • To characterize clear plaque mutants of phage HK022.
    • To establish complementation groups and construct a genetic map.
    • To investigate temperature-sensitive mutations in phage HK022.

    Main Methods:

    • Chemical mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine.
    • Isolation and characterization of clear plaque mutants.
    • Complementation tests to define cistrons (cI and cII).
    • Temperature-shift experiments to identify temperature-sensitive (cts) mutants.

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    Main Results:

    • 225 clear plaque mutants were isolated, falling into two complementation groups: cI and cII.
    • Approximately 25% of mutants were temperature-sensitive (cts).
    • A vegetative genetic map was deduced: sus4--cII-cI-sus3.
    • Isolation of cryptic lysogens from cts mutants was achieved.

    Conclusions:

    • The study successfully characterized phage HK022 clear plaque mutants.
    • Complementation analysis defined key genetic loci (cI and cII).
    • Temperature sensitivity provided insights into phage gene function and regulation.
    • A genetic map was established, contributing to understanding phage HK022 genome organization.