Related Experiment Videos
[Detection of insertion/deletion polymorphism of angiotensin converting enzyme gene in preeclampsia]
1Department of Obstetrics and Gynecology, General Hospital of Jinan Military Region, Jinan, Shandong, 250031 P.R.China.
Objective:
To establish a method for sensitive, specific and rapid detection of the angiotensin converting enzyme(ACE) genotypes and to study the relationship between the ACE gene polymorphism and preeclampsia.
Methods:
Sixty patients with preeclampsia and 76 normal pregnant women as controls were investigated. A pair of primers, for the intron 16 of ACE gene was designed. A sensitive and specific method for detecting the ACE polymorphism of the insertion/deletion was established. Determined by polymerase chain reaction (PCR),a 490bp(I) and 190bp(D) PCR product was identified,corresponding to the PCR amplification of the allele with or without the insertion.
Results:
The subjects were classified, according to the presence or absence of a 287bp insertion in intron 16 of the ACE gene, as II, DD, or heterozygotes for deletion/insertion (DI). The frequency of allele gene (0.75) and the percentage of the ACE DD genotype (65%) in the preeclampsia group were significantly higher as compared with the frequency 10.308 and the percentage (10.5%) in the control group respectively.
Conclusion:
Genotype II of ACE is a marker for reduced risk for preeclampsia and DD is a risk gene.