Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In vitro testing in pediatric food allergy

T M Herr1, P R Cook, G Highfill

  • 1Division of Otolaryngology, University of Missouri School of Medicine, Columbia, USA.

Otolaryngology--Head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery
|February 9, 1999
PubMed
Summary

In vitro testing for IgE-mediated food allergies in children is valuable. Positive results help guide treatment, even though symptom severity is similar in children with and without food allergies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Complex small-world regulatory networks emerge from the 3D organisation of the human genome.

Nature communications·2021
Same author

Nonequilibrium Chromosome Looping via Molecular Slip Links.

Physical review letters·2018
Same author

Extrusion without a motor: a new take on the loop extrusion model of genome organization.

Nucleus (Austin, Tex.)·2018
Same author

Simulating topological domains in human chromosomes with a fitting-free model.

Nucleus (Austin, Tex.)·2016
Same author

Merging drops in a Teflon tube, and transferring fluid between them, illustrated by protein crystallization and drug screening.

Lab on a chip·2015
Same author

A simple model for DNA bridging proteins and bacterial or human genomes: bridging-induced attraction and genome compaction.

Journal of physics. Condensed matter : an Institute of Physics journal·2015

Area of Science:

  • Pediatric Allergy and Immunology
  • Clinical Immunology
  • Diagnostic Testing in Allergy

Background:

  • Food allergies significantly impact pediatric morbidity.
  • Evaluating children for food allergy often involves various diagnostic methods.
  • In vitro testing is one tool used to assess potential food allergies.

Purpose of the Study:

  • To assess the value of in vitro testing in evaluating children for food allergy.
  • To compare symptom severity and improvement scores between children with and without food allergies based on in vitro test results.
  • To determine if in vitro testing is warranted in children presenting with allergic symptoms.

Main Methods:

  • A questionnaire was used to collect data from pediatric patients.
  • Patients were categorized into groups based on in vitro testing results for food allergy (positive vs. negative).

Related Experiment Videos

  • Pretreatment and symptom-improvement scores were analyzed between the groups.

    • Main Results:

    • 49% of the 77 patients tested were positive for food allergies via in vitro testing.
    • No statistically significant difference was found in pretreatment symptom scores between food allergy-positive and negative groups (55 vs. 65, p = 0.11).
    • Symptom-improvement scores also showed no significant difference between the groups (37 vs. 40, p = 0.57).

    Conclusions:

    • In vitro testing for food allergies is warranted in children with allergic symptoms.
    • Positive in vitro tests can help physicians modify treatment plans and potentially avoid oral food challenges.
    • The study highlights that while in vitro testing detects IgE levels, a negative result does not rule out non-IgE-mediated food allergies.