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Related Experiment Videos

Telomerase activity in microdissected human gliomas

R J Weil1, Y Y Wu, A O Vortmeyer

  • 1Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA.

Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc
|February 9, 1999
PubMed
Summary

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Telomerase reactivation is common in gliomas and other brain tumors, detected using tissue microdissection and quantitative assays. This finding may lead to new therapeutic targets for brain tumor treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Neuroscience

Background:

  • Improving glioma diagnosis and treatment requires understanding individual tumor cell biology.
  • Telomerase, an enzyme synthesizing telomeres, is expressed in many human tumors, including brain tumors.

Purpose of the Study:

  • To investigate the role of telomerase gain-of-function in glioma pathogenesis.
  • To determine if telomerase levels have prognostic significance in gliomas.
  • To establish a method for detecting telomerase activity in specific brain tumor cells.

Main Methods:

  • Selective tissue microdissection was used to isolate tumor cells from glioma, meningioma, and lymphoma samples.
  • Normal brain tissue served as a control.
  • Telomerase activity was assessed using electrophoretic and quantitative ELISA methods.

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Main Results:

  • All eight gliomas and the lymphoma exhibited positive telomerase activity.
  • The meningioma and normal brain tissue were negative for telomerase activity.
  • Quantitative telomerase activity did not correlate with tumor grade or predict patient outcome.

Conclusions:

  • Selective tissue microdissection combined with telomerase assays provides reliable detection of telomerase activity in brain tumors.
  • Telomerase reactivation is a frequent event in glioma tumorigenesis across all grades.
  • Targeting telomerase reactivation could offer a novel therapeutic strategy for brain tumor formation and progression.