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Penetration studies of clotrimazole from semisolid formulation using step-scan FT-IR photoacoustic spectroscopy
A Schendzielorz1, B D Hanh, R H Neubert
1Martin-Luther-University, School of Pharmacy, Institute of Pharmaceutics and Biopharmaceutics, Halle/Saale, Germany.
Purpose:
The aim of this study was to elucidate the potential use of the step-scan FT-IR photoacoustic spectroscopy (PAS) for the non-destructive determination of drug penetration into membranes.
Methods:
The penetration of clotrimazole from a 10% (w/w) suspension in Vaseline into a dodecanol-collodion acceptor membrane was studied by three methods: the step-scan FT-IR PAS with a phase modulation, a multilayer membrane system, and a modified libration model. Based on Fick's second law, the diffusion coefficient of the drug in the membrane was derived by numerical fitting of the experimental data.
Results:
The three methods applied provided almost the same diffusion coefficient D = 2.2 10(-9) cm2/s for clotrimazole in the membrane. Because of the non-destructive mode of operation, the accuracy of results obtained by FT-IR PAS is much better than that attainable by other two methods.
Conclusions:
Step-scan FT-IR photoacoustic spectroscopy in conjunction with a phase modulation is useful to determine the penetration of drug through membranes. The fact that samples can be investigated without elaborate preparation is an advantage of this spectroscopic technique.