Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nitric oxide synthase isoform expression in the developing lung epithelium

T S Sherman1, Z Chen, I S Yuhanna

  • 1Department of Pediatrics, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235, USA.

The American Journal of Physiology
|February 10, 1999
PubMed
Summary

Nitric oxide (NO) synthase isoforms are found in developing sheep lungs. These enzymes show differential expression in airway and alveolar epithelia, suggesting roles in lung development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Implementation and clinical audit of a virtual thoracic oncology MDT in a low-resource setting in Malaysia.

The Medical journal of Malaysia·2026
Same author

Ante-partum localisation of the placenta with radioactive isotope Indium (113mIN).

The Medical journal of Malaysia·2022
Same author

Giant epithelial nonparasitic splenic cyst a pre-operative diagnosis dilemma: A case report.

The Medical journal of Malaysia·2021
Same author

Relapsing retroperitoenal abscess secondary to juvenile dermatomyositis: Complexity in management.

The Medical journal of Malaysia·2020
Same author

Differences Between the East and the West in Managing Advanced-Stage Non-small Cell Lung Cancer.

Clinical oncology (Royal College of Radiologists (Great Britain))·2019
Same author

Real-world treatment patterns and outcomes in refractory metastatic colorectal cancer.

Asia-Pacific journal of clinical oncology·2019

Area of Science:

  • Pulmonary physiology
  • Developmental biology
  • Cellular biology

Background:

  • Nitric oxide (NO) is a key mediator of physiological processes in the respiratory system.
  • NO synthase (NOS) enzymes generate NO, with endothelial NOS (eNOS) expression known to be developmentally regulated in the lung.
  • Understanding NOS distribution is crucial for comprehending lung development and function.

Purpose of the Study:

  • To map the cellular distribution of eNOS expression in the developing respiratory epithelium of sheep.
  • To compare the expression patterns of eNOS with inducible NOS (iNOS) and neuronal NOS (nNOS) in the developing lung.
  • To investigate potential developmental regulation of NOS isoform expression in the lung.

Main Methods:

  • Immunohistochemistry was performed on fetal, newborn, and adult maternal sheep lungs.

Related Experiment Videos

  • Isoform-specific reverse transcription-polymerase chain reaction (RT-PCR) assays were utilized.
  • NADPH diaphorase histochemistry was employed to confirm findings.

    • Main Results:

    • eNOS and iNOS were expressed in bronchial and proximal bronchiolar epithelia, but not alveolar epithelium.
    • iNOS was also detected in terminal and respiratory bronchioles.
    • nNOS was found in epithelium at all levels, including the alveolar wall, and in airway and vascular smooth muscle.
    • The distribution patterns of all three NOS isoforms remained consistent across fetal, newborn, and adult lung tissues.

    Conclusions:

    • The three NOS isoforms (eNOS, iNOS, nNOS) are commonly expressed in proximal lung epithelium.
    • Differential expression of NOS isoforms occurs in distal lung epithelium.
    • All three NOS isoforms may contribute to epithelium-derived NO production throughout lung development.
    • NOS expression patterns are established early in lung development and are maintained into adulthood.