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Related Experiment Videos

Lysozyme in hen blood serum

Y Sato, K Watanabe

    Poultry Science
    |September 1, 1976
    PubMed
    Summary

    Hen blood serum contains lysozyme, an enzyme active against Micrococcus lysodeikticus. This serum lysozyme is a basic protein, less heat-stable than egg white lysozyme.

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    Area of Science:

    • Biochemistry
    • Immunology

    Background:

    • Lysozyme is a key enzyme in innate immunity, known for its antimicrobial properties.
    • Hen egg white is a primary source of purified lysozyme.

    Purpose of the Study:

    • To investigate the presence and characteristics of lysozyme in hen blood serum.
    • To compare serum lysozyme with hen egg white lysozyme.

    Main Methods:

    • Lytic activity assays against Micrococcus lysodeikticus.
    • Broth culture growth inhibition tests.
    • Protein purification using column chromatography and gel filtration.
    • Analysis of biochemical properties including pH stability and amino acid composition.

    Main Results:

    • Hen blood serum exhibited significant lytic activity against Micrococcus lysodeikticus.
    • Purified serum lysozyme is a basic, low molecular weight protein (approx. 15,000 Da).
    • Serum lysozyme showed higher stability at acidic pH and lower heat stability compared to egg white lysozyme.
    • Distinct amino acid composition differences were observed between serum and egg white lysozyme.

    Conclusions:

    • Hen blood serum is a source of active lysozyme with distinct properties.
    • Serum lysozyme differs in heat stability and amino acid profile from egg white lysozyme.
    • These findings contribute to understanding the distribution and characteristics of lysozyme in avian species.

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