Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enzyme induction in the uremic liver

H W Leber, L Gleumes, G Schütterle

    Kidney International. Supplement
    |June 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Uremia in male rats did not alter liver microsomal enzymes, but their activity decreased overall due to less protein. However, these enzymes can still be induced by foreign compounds under uremic conditions.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Coronary risk factors in chronic hemodialysis patients].

    Medizinische Klinik (Munich, Germany : 1983)·1996
    Same author

    Protein adsorption by artificial membrane materials under filtration conditions.

    Artificial organs·1995
    Same author

    Thrombocyte alpha-2-adrenoceptors and hypotension in hemodialyzed patients.

    Nephron·1994
    Same author

    Regulation of adrenergic receptors in the rat kidney.

    The Journal of pharmacy and pharmacology·1993
    Same author

    Monoclonal antibodies against luminal membranes of renal proximal tubules which are kidney-specific.

    Biochimica et biophysica acta·1993
    Same author

    [Atypical mycobacteriosis in immunosuppression].

    Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete·1993

    Area of Science:

    • Biochemistry
    • Toxicology
    • Physiology

    Background:

    • Uremia, a condition resulting from kidney failure, can affect various physiological processes.
    • Liver microsomal enzymes play a crucial role in metabolizing both endogenous and exogenous compounds.
    • Investigating the impact of uremia on liver microsomal enzyme activity is essential for understanding drug metabolism and toxicity in kidney disease.

    Purpose of the Study:

    • To investigate the in vitro activity of liver microsomal enzymes in male rats 45 days after subtotal nephrectomy (uremia) or sham-operation.
    • To determine if uremia itself induces or inhibits specific microsomal enzymes.
    • To assess the inducibility of these enzymes by foreign compounds in the presence of uremia.

    Main Methods:

    • Subtotal nephrectomy or sham-operation was performed on male rats.

    Related Experiment Videos

  • Microsomal enzyme activities (esterases, glucuronyltransferases, mixed-function oxidation system) were measured in vitro.
  • Enzyme activities were assessed per milligram of microsomal protein and for the whole liver.
  • The effect of di-(2-ethylhexyl)-phthalate on liver and enzyme parameters was evaluated.

    • Main Results:

    • Uremic rats showed normal activities of two esterases and two glucuronyltransferases per milligram of microsomal protein.
    • The mixed-function oxidation system exhibited lower activity per milligram of protein in uremic rats.
    • Despite decreased microsomal protein content in uremic livers, phenolphthalein glucuronidation remained normal per whole liver.
    • Di-(2-ethylhexyl)-phthalate induced liver weight, microsomal protein, and aminopyrine demethylation in uremic rats, but not in controls.

    Conclusions:

    • Uremia itself does not induce liver microsomal enzymes.
    • Liver microsomal enzymes retain their inducibility by foreign compounds even under uremic conditions.
    • These findings have implications for drug metabolism and toxicity assessment in patients with kidney disease.