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Human effector memory T cells express CD86: a functional role in naive T cell priming
P Jeannin1, N Herbault, Y Delneste
1Centre d'Immunologie Pierre Fabre, Saint-Julien en Genevois, France. pascale.jeannin@pierre-fabre.com
Journal of Immunology (Baltimore, Md. : 1950)
|February 11, 1999
Summary
Human memory effector T cells express functional CD86, a glycoprotein that costimulates naive T cell responses. This finding clarifies the role of CD86 beyond antigen-presenting cells in immune activation.
Area of Science:
- Immunology
- Cell Biology
Background:
- CD86 on antigen-presenting cells (APCs) is crucial for T cell activation.
- The expression and function of CD86 on T cells remain controversial.
Purpose of the Study:
- To investigate the phenotype and function of CD86-expressing T cells derived from human peripheral blood mononuclear cells (PBMCs).
Main Methods:
- Analysis of T cell phenotype using flow cytometry.
- Assessment of biological activity through T cell proliferation assays and cytokine production measurements.
- Functional blocking with anti-CD86 monoclonal antibodies (mAbs).
Main Results:
- CD86 expression on T cells is induced by prolonged stimulation (CD3 and IL-2R) and diminishes upon quiescence.
- CD86+ T cells are identified as memory effector cells expressing CD45RO, activation markers (CD25, CD54, HLA-DR), CD30, CD40-ligand, and CD70.
- These cells produce IFN-gamma and can enhance naive T cell proliferation and IFN-gamma production, an effect blocked by anti-CD86 mAbs.
Conclusions:
- Human memory effector T cells express a functional CD86 molecule.
- This functional CD86 on T cells can provide costimulatory signals to naive T cells, influencing their response.