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Related Experiment Videos

Expression patterns of folate binding proteins one and two in the developing mouse embryo

R C Barber1, G D Bennett, K A Greer

  • 1Department of Veterinary Anatomy and Public Health, Texas A&M University, College Station, Texas, 77843-4458, USA.

Molecular Genetics and Metabolism
|February 12, 1999
PubMed
Summary

Murine folate binding proteins (FBP1 and FBP2) show specific expression patterns during embryonic development. FBP1 is highly expressed in embryonic placental tissue, suggesting a role in placental development and neural tube morphogenesis.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Folate binding proteins (FBPs) are crucial for folate transport.
  • Murine FBPs (FBP1 and FBP2) are homologues of human folate receptors.
  • Nomenclature of folate receptor genes has caused confusion.

Purpose of the Study:

  • To determine the expression patterns of murine folate binding proteins 1 and 2 (FBP1 and FBP2) during embryonic development.
  • To clarify the roles of FBP1 and FBP2 in mouse embryogenesis.
  • To resolve nomenclature issues regarding folate receptor gene family.

Main Methods:

  • Ribonuclease protection assay (RPA) was used to analyze mRNA expression.
  • Tissue samples were collected from anterior neural tube and term placenta (maternal and fetal).

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  • Expression levels were compared across different embryonic developmental stages and tissues.

    • Main Results:

    • FBP1 mRNA expression peaked in term placental tissue, particularly in the embryonic-derived portion.
    • FBP2 mRNA expression showed no significant statistical differences between tissues or time points.
    • Both FBP1 and FBP2 showed a slight, non-significant elevation during neural tube closure (Gestational Days 8-10).

    Conclusions:

    • The observed expression patterns align with those expected for murine homologues of human folate receptors alpha and beta.
    • The expression of FBP1 and FBP2 during neural tube closure suggests their involvement in neural tube morphogenesis.
    • These findings contribute to resolving nomenclature confusion and highlight the functional significance of these genes in embryonic development.