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Apoptosis in early ocular morphogenesis in the mouse

L K Laemle1, M Puszkarczuk, R N Feinberg

  • 1Department of Anatomy, Cell Biology and Injury Sciences, UMDNJ, New Jersey Medical School, Newark 07103, USA. laemlelb@umdnj.edu

Brain Research. Developmental Brain Research
|February 12, 1999
PubMed
Summary

Apoptosis, a form of programmed cell death, is crucial for normal eye development in ZRDCT-N mice. This study maps the timing and location of apoptotic cells during ocular morphogenesis.

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Area of Science:

  • Developmental Biology
  • Ophthalmology
  • Cell Biology

Background:

  • Eye development involves intricate interactions between tissues, extracellular matrix, and growth factors.
  • Cellular development can lead to differentiation or programmed cell death, primarily apoptosis or necrosis.
  • Understanding cell death mechanisms is vital for studying normal and abnormal ocular development.

Purpose of the Study:

  • To identify the mechanism of cell death during ocular morphogenesis in ZRDCT-N mice.
  • To map the spatial and temporal distribution of apoptotic cells during eye development.
  • To establish a foundation for investigating abnormal ocular development.

Main Methods:

  • Mouse embryos (E8.5-E11.5) were processed using paraffin embedding and sectioning.

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  • Tissues were stained with hematoxylin or subjected to terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay.
  • Computerized image analysis and 3-D reconstructions were utilized to map apoptotic cell distribution.

    • Main Results:

    • Apoptosis was identified as the primary mechanism of cell death during ocular morphogenesis in ZRDCT-N mice.
    • The study mapped the precise timing and location of apoptotic cells throughout embryonic eye development.
    • Detailed 3-D reconstructions provided insights into the role of apoptosis in shaping ocular structures.

    Conclusions:

    • Apoptosis plays a significant role in normal ocular morphogenesis.
    • This research provides essential data for future studies on congenital eye abnormalities.
    • The findings highlight the importance of programmed cell death in establishing proper eye anatomy.