Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Microbial breakdown of caffeine (author's transl)]

R Blecher

    Zentralblatt Fur Bakteriologie, Parasitenkunde, Infektionskrankheiten Und Hygiene. Erste Abteilung Originale. Reihe B: Hygiene, Praventive Medizin
    |July 1, 1976
    PubMed
    Summary

    Pseudomonas putida bacteria can metabolize caffeine as their only source of carbon and nitrogen. This process involves breaking down caffeine into simpler compounds like urea and glyoxylic acid for energy.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Isolated unilateral facet dislocation of the lumbosacral junction.

    The Journal of bone and joint surgery. British volume·2010
    Same author

    [Time for an efficiency assessment system for patients with schizophrenia].

    Lakartidningen·1991
    Same author

    Bacterial degradation of aminopyrine.

    Xenobiotica; the fate of foreign compounds in biological systems·1981
    Same author

    The metabolism of caffeine by a Pseudomonas putida strain.

    Hoppe-Seyler's Zeitschrift fur physiologische Chemie·1977

    Area of Science:

    • Microbiology
    • Biochemistry

    Context:

    • Caffeine is a widely consumed stimulant with potential environmental persistence.
    • Understanding microbial degradation pathways is crucial for bioremediation.

    Purpose:

    • To isolate and identify a bacterium capable of utilizing caffeine as a sole carbon and nitrogen source.
    • To elucidate the metabolic pathway for caffeine breakdown by the identified bacterium.

    Summary:

    • A soil bacterium, identified as Pseudomonas putida, was isolated for its ability to grow aerobically using caffeine as its sole carbon and nitrogen source.
    • The degradation pathway initiates with stepwise demethylation of caffeine, producing formaldehyde and N-methyl-purines, ultimately leading to xanthine.
    • Xanthine is further metabolized through intermediates like uric acid and allantoin, yielding urea and glyoxylic acid, which serves as the bacterium's carbon source.

    Impact:

    • Identifies a specific bacterial species and its metabolic capabilities for caffeine degradation.
    • Provides insights into the biochemical pathways involved in purine catabolism.
    • Suggests potential applications for Pseudomonas putida in bioremediation of caffeine-contaminated environments.

    Related Experiment Videos