Related Experiment Video
Updated: Aug 12, 2026
11:31
Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols
Published on: January 2, 2013
Summary
The study found that PIGPAd fluid effectively regenerated erythrocytes, maintaining adenosine triphosphate (ATP) and 2,3-diphosphoglycerate (2,3-DPG) levels up to 70 days. This suggests that erythrocyte storage duration can be extended to 70 days.
Area of Science:
- Biochemistry
- Hematology
- Cellular Biology
Context:
- Erythrocyte storage is crucial for transfusion medicine.
- Current storage limitations necessitate frequent replenishment of blood supplies.
- Investigating methods to extend erythrocyte viability is of significant clinical interest.
Purpose:
- To evaluate the efficacy of three rejuvenating fluids (PIGP, PIGPA, PIGPAd) in restoring biochemical properties of stored erythrocytes.
- To determine the optimal fluid for extending erythrocyte storage duration.
- To assess the impact of rejuvenation on erythrocyte ATP, 2,3-DPG, osmotic resistance, recovery, and free hemoglobin.
Summary:
- Erythrocytes stored for 70 days at 4°C were incubated with PIGP, PIGPA, or PIGPAd fluids at 37°C.
- Biochemical parameters including ATP, 2,3-DPG, osmotic resistance, recovery, and free hemoglobin were measured immediately post-incubation and after 48 hours.
- PIGPAd fluid demonstrated superior regenerative properties, maintaining elevated ATP and 2,3-DPG levels until day 70.
Impact:
- The findings suggest that PIGPAd fluid can significantly enhance erythrocyte preservation.
- Extending erythrocyte storage to 70 days is feasible, potentially reducing transfusion-related costs and improving blood supply management.
- This research contributes to optimizing blood banking practices and ensuring the availability of viable red blood cells for patients.
Related Concept Videos
01:14Erythropoiesis
Red blood cells (RBCs) transport oxygen to all body tissues. These cells survive only for 120 days and then need to be replenished. Erythropoiesis is the process of RBC production. In healthy individuals, erythropoiesis ensures all tissues are amply supplied with oxygen. In addition, blood loss due to injury leads to a drop in the physiological oxygen level that will cause erythropoiesis. Any defect in erythropoiesis leads to several physiological disorders, including thalassemia, anemia, and...
01:29Structure and Function of Erythrocytes
There are between 4.2 and 6 million erythrocytes, also known as red blood cells, in every microliter of blood. These cells are small, flattened biconcave discs with centers that are depressed.
The erythrocyte plasma membrane is associated with proteins such as spectrin, which forms a flexible cytoplasmic meshwork. This meshwork allows erythrocytes to twist, turn, become cup-shaped, and regain their biconcave shape as they pass through narrow capillaries. Additionally, erythrocytes can form...
The erythrocyte plasma membrane is associated with proteins such as spectrin, which forms a flexible cytoplasmic meshwork. This meshwork allows erythrocytes to twist, turn, become cup-shaped, and regain their biconcave shape as they pass through narrow capillaries. Additionally, erythrocytes can form...
01:22Lifecycle of Erythrocytes
Erythrocytes, also known as red blood cells, constantly move through blood capillaries. As a result, they damage their plasma membrane due to the continuous friction. Typically, after 100 to 120 days, erythrocytes become rigid and fragile as they wear out. As they pass through small vessels in the spleen and liver, they can get trapped and break apart into fragments.
The resident phagocytic macrophages deal with these damaged cells by engulfing them and separating their globin and heme groups.
The resident phagocytic macrophages deal with these damaged cells by engulfing them and separating their globin and heme groups.
01:27Disorders of Erythrocytes
Disorders of erythrocytes, or red blood cells (RBCs), include a range of conditions affecting their number, shape, or function.
Erythrocyte disorders can be broadly categorized into two main types: anemic and polycythemic conditions.
A low oxygen-carrying capacity of the blood due to the loss, lower production, or destruction of erythrocytes is termed anemia. Hemorrhagic anemia, for example, occurs when bleeding from an external wound or internal ulcer reduces erythrocyte counts.
On the other...
Erythrocyte disorders can be broadly categorized into two main types: anemic and polycythemic conditions.
A low oxygen-carrying capacity of the blood due to the loss, lower production, or destruction of erythrocytes is termed anemia. Hemorrhagic anemia, for example, occurs when bleeding from an external wound or internal ulcer reduces erythrocyte counts.
On the other...
01:24Factors Affecting Erythropoiesis
The cardiovascular system regulates the number of erythrocytes in the bloodstream to ensure optimal oxygen transport. It also prevents over-proliferation of these cells, which helps to maintain blood viscosity and flow rate.
Several factors influence the erythrocyte production rate, with tissue oxygen level being among the most critical. Intense exercise or high altitudes can cause tissue hypoxia, which triggers the kidneys to release more erythropoietin (EPO) into the bloodstream.
EPO then...
Several factors influence the erythrocyte production rate, with tissue oxygen level being among the most critical. Intense exercise or high altitudes can cause tissue hypoxia, which triggers the kidneys to release more erythropoietin (EPO) into the bloodstream.
EPO then...
01:27Oxygen Transport in the Blood
Hemoglobin (Hb) is a crucial molecule in the human body, consisting of four polypeptide chains, each bound to an iron-containing heme group. This unique structure enables hemoglobin to bind to oxygen, with each molecule capable of combining with four molecules of oxygen, leading to rapid and reversible oxygen loading. When fully loaded with oxygen, it is called oxyhemoglobin, while hemoglobin that has released oxygen is called reduced hemoglobin or deoxyhemoglobin. As hemoglobin binds oxygen,...