Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Skeletal malformations induced by mitomycin C in chick embryos

J D Singh, S Singh

    Acta Orthopaedica Scandinavica
    |October 1, 1976
    PubMed
    Summary

    Mitomycin C exposure in chick embryos caused significant skeletal malformations, particularly affecting limb development. This study highlights the teratogenic effects of mitomycin C on embryonic skeletal development.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Non-transferrin-bound iron species in the serum of hypotransferrinaemic mice.

    Biochimica et biophysica acta·1992
    Same author

    "Pseudo-resistant" malaria in tropical countries.

    Lancet (London, England)·1992
    Same author

    Effect of PEG 400, ethanol and laurocapram (Azone) on the transport of testosterone through rat skin.

    Die Pharmazie·1992
    Same author

    Effect upon plaque formation and gingivitis of a triclosan/copolymer/fluoride dentifrice: a 6-month clinical study.

    American journal of dentistry·1992
    Same author

    Asymptomatic Salmonella senftenberg carriage in a neonatal ward.

    The Journal of hospital infection·1992
    Same author

    Cutaneous toxicity of sodium lauryl sulphate, nickel, and their combination in guinea pigs: biochemical and histopathological observations.

    Bulletin of environmental contamination and toxicology·1992

    Area of Science:

    • Developmental Biology
    • Teratology
    • Embryology

    Background:

    • Mitomycin C is a chemotherapeutic agent with known mutagenic properties.
    • Embryonic development is sensitive to environmental factors and chemical exposures.
    • Understanding drug effects on skeletal development is crucial for risk assessment.

    Purpose of the Study:

    • To investigate the teratogenic effects of mitomycin C on chick embryo skeletal development.
    • To identify specific skeletal malformations induced by mitomycin C exposure at different incubation stages.

    Main Methods:

    • 180 chick embryos were administered mitomycin C at 72, 96, and 120 hours of incubation.
    • Skeletal malformations of the axial and appendicular skeleton were assessed.
    • Quantitative analysis of limb bone lengths was performed and compared to controls.

    Main Results:

    • Mitomycin C induced defects in the axial skeleton (ribs, vertebrae, beak, crania) and appendicular skeleton.
    • Lower limb defects included absent phalanges, and bent/shortened femur, fibula, tibia, and metatarsus.
    • Upper limb defects included absent phalanges and curved scapulae, with significant reduction in femur, tibia, and metatarsus length (P < 0.001), especially after 96-hour treatment.

    Conclusions:

    • Mitomycin C exposure during embryonic development leads to a spectrum of skeletal malformations in chick embryos.
    • The timing of exposure influences the severity and type of skeletal defects observed.
    • These findings underscore the teratogenic potential of mitomycin C and its impact on limb morphogenesis.

    Related Experiment Videos