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Related Experiment Videos

Intracellular calcium release channel expression during embryogenesis

N Rosemblit1, M C Moschella, E Ondriašová

  • 1Molecular Cardiology Program, Divisions of Circulatory Physiology and Cardiology, Department of Medicine, New York, New York, 10032, USA.

Developmental Biology
|February 13, 1999
PubMed
Summary

Intracellular calcium (Ca2+) release channels, inositol 1,4, 5-trisphosphate receptors (IP3R) and ryanodine receptors (RyR), shift during development. Early embryos widely express IP3R, while mature excitable cells primarily use RyR for Ca2+ signaling.

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Area of Science:

  • Cellular Biology
  • Developmental Biology
  • Molecular Physiology

Background:

  • Intracellular calcium (Ca2+) release is crucial for diverse cellular signaling pathways.
  • Two primary channels mediate Ca2+ release: inositol 1,4, 5-trisphosphate receptors (IP3R) and ryanodine receptors (RyR).
  • The roles and expression patterns of these channels during development are not fully elucidated.

Purpose of the Study:

  • To investigate the developmental expression and functional roles of IP3R and RyR in murine embryos.
  • To understand the transition in Ca2+ release channel usage during organogenesis and cellular differentiation.
  • To correlate channel expression with specific cellular functions like proliferation, apoptosis, and muscle contraction.

Main Methods:

  • Analysis of IP3R mRNA expression in murine embryonic tissues at various developmental stages.

Related Experiment Videos

  • Assessment of functional IP3-gated Ca2+ release channels.
  • Characterization of RyR channel expression and activation mechanisms (voltage-dependent, Ca2+-induced Ca2+ release) during development.

    • Main Results:

    • IP3R mRNA and functional IP3-gated Ca2+ channels are broadly expressed in early murine embryos across most tissues.
    • During organogenesis, RyR channels become more prominent in specific cell types, alongside diverse Ca2+ release triggers.
    • Excitable cells, such as cardiac and skeletal muscle, predominantly utilize RyRs in later developmental stages, while IP3Rs remain expressed in most cell types.

    Conclusions:

    • A developmental switch occurs from primarily IP3R-mediated to both IP3R-mediated and IP3R-independent (RyR) Ca2+ release pathways.
    • This transition is linked to the distinct roles of IP3R in proliferation/apoptosis and RyR in functions like muscle contraction.
    • The differential expression and regulation of IP3R and RyR are critical for proper embryonic development and cellular specialization.