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Updated: Jul 24, 2026

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Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
Published on: September 30, 2016
Identification of contaminating fungal DNA sequences in Zymolyase
1Hygiene Institute, Children's Hospital, University of Heidelberg, Heidelberg, Germany. dagmar_rimek@ukl.uniheidelberg.de
Journal of Clinical Microbiology
|February 13, 1999
Abstract:
When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu stricto complex and Kluyveromyces lodderae. Lyticase, lysing enzymes, and proteinase K appeared to be free from fungal DNA.

