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Hepatic cell integrity in hypodynamic states

R P Carlson, A M Lefer

    The American Journal of Physiology
    |November 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Simulated shock conditions, especially hypoxia, significantly impair liver function and cell integrity in cats. These effects, including enzyme release and reduced phagocytosis, worsen the shock state.

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    Area of Science:

    • Hepatology
    • Physiology
    • Shock Pathophysiology

    Background:

    • Liver integrity is crucial during circulatory shock.
    • Understanding the impact of hypodynamic states on liver function is essential.

    Purpose of the Study:

    • To investigate liver integrity changes in isolated perfused cat livers under simulated shock conditions.
    • To differentiate the effects of hypoxia, acidosis, and ischemia on liver function.

    Main Methods:

    • Isolated perfused cat liver model.
    • Simulated shock conditions (hypoxia, acidosis, ischemia) individually and combined.
    • Measurement of carbon clearance, lactic acid dehydrogenase (LDH), and cathepsin D activities.

    Main Results:

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  • Combined stimuli reduced carbon clearance by 51% and elevated LDH and cathepsin D.
  • Hypoxia had a more severe impact than acidosis or ischemia alone.
  • Hypoxia and ischemia increased free cathepsin D in liver tissue; Kupffer and parenchymal cell integrity was impaired between 60-90 minutes.

    • Conclusions:

    • The liver is stable for 60 minutes under hypoperfusion but sensitive to hypoxia.
    • Hypoxia, acidosis, and ischemia release hepatic enzymes and depress reticuloendothelial system function.
    • These changes exacerbate the shock state, highlighting hypoxia as a key detrimental factor.