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Related Experiment Videos

An encyclopedia of mouse genes

M Marra1, L Hillier, T Kucaba

  • 1Washington University Genome Sequencing Center, St. Louis, Missouri 63108, USA. mmarra@alu.wustl.edu

Nature Genetics
|February 13, 1999
PubMed
Summary

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Researchers created a comprehensive database of mouse expressed sequence tags (ESTs) to accelerate gene discovery and analysis in mammalian development research. This extensive EST collection significantly enhances the utility of the mouse as a model organism.

Area of Science:

  • Developmental Biology
  • Genomics
  • Bioinformatics

Background:

  • The laboratory mouse is a key model for mammalian development studies.
  • Existing molecular tools and genetic analysis capabilities are powerful but can be enhanced.
  • A need exists for rapid gene access and comprehensive developmental data.

Purpose of the Study:

  • To establish a large database of expressed sequence tags (ESTs) for the mouse.
  • To provide rapid access to gene information for research.
  • To enable studies from early developmental stages using cDNA libraries.

Main Methods:

  • Initiated a program to generate mouse ESTs starting March 1996.
  • Focused on 5' end sequences from directionally cloned, oligo-dT primed cDNA libraries.

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  • Generated and annotated 352,040 sequences by October 1998.

    • Main Results:

    • Developed a comprehensive database of mouse ESTs.
    • The database comprised 93% of all available mouse ESTs in dbEST by October 1998.
    • Demonstrated the versatility of EST data for various applications.

    Conclusions:

    • The comprehensive mouse EST database significantly enhances the mouse model system.
    • EST data are crucial for gene identification, mapping, and genome annotation.
    • This resource supports diverse applications including microarray development and disease gene discovery.