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Updated: Aug 4, 2026

Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice
08:57

Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice

Published on: October 5, 2017

[Cross resistance in tumor cells and staphylococci with respect to anthracycline-group antineoplastic antibiotics]

V A Shorin, L A Averbukh

    Antibiotiki
    |October 1, 1976
    PubMed

    Insights

    Tumor cells and bacteria developed partial resistance to adriamycin when exposed to rubomycin. Karminomycin sensitivity remained largely unchanged, suggesting distinct drug resistance mechanisms.

    Area of Science:

    • Microbiology
    • Pharmacology
    • Oncology

    Context:

    • Investigating cross-resistance patterns in microbial and tumor cells exposed to anthracycline antibiotics.
    • Examining the impact of rubomycin and adriamycin on the sensitivity of Fisher lymphadenosis cells (L-5178) and Staphylococcus aureus.
    • Assessing the development of resistance to karminomycin in conjunction with other anthracyclines.

    Purpose:

    • To determine the cross-resistance profiles between rubomycin, adriamycin, and karminomycin in bacterial and tumor cell lines.
    • To elucidate the mechanisms underlying acquired drug resistance, potentially involving cell membrane permeability changes.

    Summary:

    • Fisher lymphadenosis cells and Staphylococcus aureus strains resistant to rubomycin exhibited partial cross-resistance to adriamycin.
    • Staphylococcus aureus showed significant decreases in sensitivity to adriamycin (67-fold) and rubomycin (4-fold) after serial passages, while karminomycin sensitivity remained relatively stable (1.5-fold decrease).
    • Extended passages (22) of Staphylococcus aureus to karminomycin, rubomycin, and adriamycin resulted in substantial resistance increases (16 to 67-fold), with adriamycin resistance being more pronounced.
    • Observed resistance patterns suggest that alterations in cell membrane permeability play a role in the differential drug responses.

    Impact:

    • Provides insights into the complex mechanisms of multidrug resistance in both microbial and cancer cells.
    • Informs the development of more effective chemotherapy strategies by understanding cross-resistance patterns of anthracycline antibiotics.
    • Highlights the potential for differential resistance development, guiding the selection of alternative therapeutic agents like karminomycin in certain contexts.

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