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[About the differentiation of antibodies against the connective tissue (author's transl)]
Unfixed cryostat sections of an organ block comprising the liver, stomach, and kidney of rats as well as the kidney of mice were used as antigenic substrate for detecting antibodies against connective tissues by indirect immunofluorescence. There were found more than 14 different fluorescent patterns. Two types could be distinguished easily: The "type 1" shows a clear fluorescence of fine perivascular fibres and a faint fluorescence of vascular endothelium in the liver and in the kidney. The sinusoids are negative. The "type 2" is characterized by a bright and broad fluorescence of the sinusoids and of the portal tract without perivascular staining of the liver. On the sections of the kidney a fluorescence of vascular endothelium, of the tubular basement membrane or of the peritubular connective tissue fibrils is typical.
Unfixed cryostat sections of an organ block comprising the liver, stomach, and kidney of rats as well as the kidney of mice were used as antigenic substrate for detecting antibodies against connective tissues by indirect immunofluorescence. There were found more than 14 different fluorescent patterns. Two types could be distinguished easily: The "type 1" shows a clear fluorescence of fine perivascular fibres and a faint fluorescence of vascular endothelium in the liver and in the kidney. The sinusoids are negative. The "type 2" is characterized by a bright and broad fluorescence of the sinusoids and of the portal tract without perivascular staining of the liver. On the sections of the kidney a fluorescence of vascular endothelium, of the tubular basement membrane or of the peritubular connective tissue fibrils is typical.