Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

125I-labelling = 125I-Labelling

M J Murphy

    The Biochemical Journal
    |November 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed a simple method to label erythropoietin (EPO) with iodine-125. This technique rapidly produced highly radioactive EPO without affecting its in vivo biological activity, crucial for hormone research.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Validation of a force transducer-embedded platform as an alternative to handles in weight perception research.

    Behavior research methods·2025
    Same author

    KBG syndrome mimicking genetic generalized epilepsy.

    Epilepsy & behavior reports·2022
    Same author

    A mixed methods pilot and feasibility open trial of internet-delivered cognitive behaviour therapy (<i>iCanADAPT Advanced</i>) for people with advanced cancer with depression and/or anxiety.

    Internet interventions·2021
    Same author

    Randomised controlled trial of internet-delivered cognitive behaviour therapy for clinical depression and/or anxiety in cancer survivors (iCanADAPT Early).

    Psycho-oncology·2019
    Same author

    A phantom study of the performance of model-based iterative reconstruction in low-dose chest and abdominal CT: When are benefits maximized?

    Radiography (London, England : 1995)·2018
    Same author

    Q-switched 532-nm laser energy causes significant vascular damage in the capillary plexus: how does this affect laser tattoo removal?

    The British journal of dermatology·2017
    Same journal

    Mechanistic insights into acetylated histone recognition by the CECR2 bromodomain.

    The Biochemical journal·2026
    Same journal

    Nanobodies against Plasmodium adhesins that block receptor engagement and malaria parasite invasion.

    The Biochemical journal·2026
    Same journal

    Persistence without turnover: the RhoG G12E mutant highlights the role of nucleotide cycling in RhoG signaling.

    The Biochemical journal·2026
    Same journal

    Alternative Splicing of Rice Chloroplastic CuZn Superoxide Dismutase, OsCSD2: Impact on expression and protein characteristics.

    The Biochemical journal·2026
    Same journal

    Difference and similarity between the ubiquitous secretory pathway Ca2+-ATPases, SERCA2b, and SPCA1a.

    The Biochemical journal·2026
    Same journal

    A molecular perspective on dimethylarginine dimethylaminohydrolases structure and function.

    The Biochemical journal·2026
    See all related articles

    Area of Science:

    • Biochemistry
    • Endocrinology
    • Radioisotopic labeling

    Background:

    • Erythropoiesis, the production of red blood cells, is regulated by the hormone erythropoietin (EPO).
    • Accurate measurement of EPO requires radiolabeled preparations.
    • Previous labeling methods were complex or compromised EPO integrity.

    Purpose of the Study:

    • To develop a rapid and simple method for radioiodination of erythropoietin.
    • To produce highly radioactive erythropoietin preparations.
    • To ensure the biological integrity of labeled erythropoietin for in vivo assays.

    Main Methods:

    • Utilized the catalytic activity of lactoperoxidase for iodination.
    • Employed glucose oxidase to generate hydrogen peroxide (H2O2).

    Related Experiment Videos

  • Performed in vivo assays to confirm biological activity of the labeled hormone.

    • Main Results:

    • Successfully labeled erythropoietin with iodine-125 (125I).
    • Achieved high specific radioactivity in the hormone preparations.
    • Demonstrated that the labeling method did not significantly alter EPO's biological integrity in vivo.

    Conclusions:

    • The described method provides a rapid, simple, and effective way to radioiodinate erythropoietin.
    • This technique yields high-quality labeled EPO suitable for biological studies.
    • The preservation of biological integrity is a key advantage for in vivo research.