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Polypyrimidine sequences found in eukaryotic DNA have been conserved during evolution

N A Straus, H C Birnboim

    Biochimica Et Biophysica Acta
    |December 13, 1976
    PubMed
    Summary
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    Eukaryotic DNA contains abundant long pyrimidine tracts that are conserved and transcribed, suggesting a crucial role in cellular function. These sequences hybridize to eukaryotic DNA but not prokaryotic DNA, indicating evolutionary significance.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Evolutionary Biology

    Background:

    • L-cell DNA exhibits an unusual abundance of long pyrimidine tracts.
    • The evolutionary origin and function of these DNA sequences remain largely unexplored.

    Purpose of the Study:

    • To investigate the hybridization properties of L-cell DNA polypyrimidines.
    • To determine the evolutionary conservation and transcriptional activity of these sequences.

    Main Methods:

    • Hydroxyapatite chromatography was used to analyze DNA hybridization.
    • Thermal stability assays assessed the fidelity of hybrid formation.
    • Hybridization with cellular RNA investigated transcriptional activity.

    Main Results:

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  • L-cell polypyrimidines extensively hybridized to reiterated DNA in diverse eukaryotes, but not prokaryotes.
  • Hybridization was sequence-specific, not a general property of polypyrimidines.
  • Polypyrimidine-eukaryotic DNA hybrids showed higher thermal stability than total DNA hybrids, indicating sequence conservation.
  • These tracts are transcribed, reacting with cellular RNA.

    • Conclusions:

    • Long pyrimidine tracts in L-cell DNA are a conserved feature across eukaryotes.
    • These sequences are transcribed and likely play a significant functional role in eukaryotic cells.
    • Their evolutionary conservation suggests an important, though currently unknown, function.