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Related Experiment Videos

Error introduced by small reflection coefficients in permeability constansts obtained by hemolysis

E L Coe

    Biochimica Et Biophysica Acta
    |December 2, 1976
    PubMed
    Summary

    This study revises theoretical calculations for nonelectrolyte permeability constants in red blood cells by incorporating the reflection coefficient (sigma). New equations provide more accurate permeability ratios (ro) than the original Jacobs method, especially when sigma is less than 1.

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    Biochimica et biophysica acta·1978

    Area of Science:

    • Biophysics
    • Cell Physiology
    • Membrane Transport

    Background:

    • M.H. Jacobs' 1933 work provided a theoretical basis for calculating nonelectrolyte permeability constants in erythrocytes using hemolysis times.
    • Jacobs' derivation assumed a reflection coefficient (sigma) of 1, which is often not the case (0 < sigma < 1).

    Purpose of the Study:

    • To revise Jacobs' theoretical derivation to include a reflection coefficient (sigma) less than 1.
    • To establish a method for calculating accurate solute permeability constants (omega) and osmotic flow (Lpcs) in erythrocytes.
    • To compare the revised permeability ratio (ro) with the apparent ratio (r) derived from the original Jacobs approach.

    Main Methods:

    • Comparison of Jacobs' original derivation with revised equations incorporating sigma.

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  • Mathematical derivation of the relationship between the apparent ratio (r) and the true ratio (ro).
  • Application of corrections for sigma < 1 to permeability constants of ethylene glycol, glycerol, and propanol in bovine erythrocytes.

    • Main Results:

    • The true permeability ratio (ro) can be expressed as a function of the apparent ratio (r) and sigma.
    • Simpler approximations for ro are provided for large (r > 10) or small (r < 0.1) values of r, with <20% error if sigma > 0.4.
    • Predicted sigma values of 0.7 and 0.6 for monoacetin and diacetin, respectively, based on deviations in their permeability constants.

    Conclusions:

    • The revised theoretical framework accurately accounts for the reflection coefficient in calculating nonelectrolyte permeability constants.
    • The study provides a more precise method for determining erythrocyte membrane permeability.
    • The findings offer insights into the membrane transport properties of various nonelectrolytes.