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The Biochemical Journal|February 15, 1992
In defence of the general validity of the Cha method of deriving rate equations. The importance of explicit recognition of the thermodynamic box in enzyme kineticsC M Topham, K BrocklehurstMethods (San Diego, Calif.)|June 1, 2001
Kinetic and titration methods for determination of active site contents of enzyme and catalytic antibody preparationsK Brocklehurst, M Resmini, C M TophamThe Biochemical Journal|December 1, 1988
Supracrystallographic resolution of interactions contributing to enzyme catalysis by use of natural structural variants and reactivity-probe kineticsK Brocklehurst, S M Brocklehurst, D Kowlessur, et al.The Biochemical Journal|November 15, 1991
Structure-function relationships in the cysteine proteinases actinidin, papain and papaya proteinase omega. Three-dimensional structure of papaya proteinase omega deduced by knowledge-based modelling and active-centre characteristics determined by two-hydronic-state reactivity probe kinetics and kinetics of catalysisC M Topham, E Salih, C Frazao, et al.The Biochemical Journal|February 15, 1993
Ionization characteristics of the Cys-25/His-159 interactive system and of the modulatory group of papain: resolution of ambiguity by electronic perturbation of the quasi-2-mercaptopyridine leaving group in a new pyrimidyl disulphide reactivity probeG W Mellor, E W Thomas, C M Topham, et al.The Biochemical Journal|March 15, 1990
Dependence of the P2-S2 stereochemical selectivity of papain on the nature of the catalytic-site chemistry. Quantification of selectivity in the catalysed hydrolysis of the enantiomeric N-acetylphenylalanylglycine 4-nitroanilidesD Kowlessur, E W Thomas, C M Topham, et al.The Biochemical Journal|March 15, 1990
A re-appraisal of the structural basis of stereochemical recognition in papain. Insensitivity of binding-site-catalytic-site signalling to P2-chirality in a time-dependent inhibitionW Templeton, D Kowlessur, E W Thomas, et al.Journal of Theoretical Biology|July 11, 2000
The kinetic basis of a general method for the investigation of active site content of enzymes and catalytic antibodies: first-order behaviour under single-turnover and cycling conditionsC M Topham, S Gul, M Resmini, et al.The Biochemical Journal|April 15, 1989
The interplay of electrostatic fields and binding interactions determining catalytic-site reactivity in actinidin. A possible origin of differences in the behaviour of actinidin and papainD Kowlessur, M O'Driscoll, C M Topham, et al.The Biochemical Journal|March 15, 1989
Identification of signalling and non-signalling binding contributions to enzyme reactivity. Alternative combinations of binding interactions provide for change in transition-state geometry in reactions of papainD Kowlessur, C M Topham, E W Thomas, et al.Pageof 11