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FEBS Letters|April 12, 2003
PCR-linked in vitro expression: a novel system for high-throughput construction and screening of protein librariesSuang Rungpragayphan, Hideo Nakano, Tsuneo YamaneMethods in Molecular Biology (Clifton, N.J.)|July 20, 2007
SIMPLEX: single-molecule PCR-linked in vitro expression: a novel method for high-throughput construction and screening of protein librariesSuang Rungpragayphan, Tsuneo Yamane, Hideo NakanoNucleic Acids Research|November 25, 2003
Exhaustive identification of interaction domains using a high-throughput method based on two-hybrid screening and PCR-convergence: molecular dissection of a kinetochore subunit Spc34pAkinori Ikeuchi, Yoko Sasaki, Yasuaki Kawarasaki, et al.Journal of Bioscience and Bioengineering|October 20, 2005
Cloning and in vitro and in vivo expression of plant glutathione S-transferase zeta class genesDefu Chen, Yasuaki Kawarasaki, Hideo Nakano, et al.Journal of Molecular Biology|August 6, 2003
Inverting enantioselectivity of Burkholderia cepacia KWI-56 lipase by combinatorial mutation and high-throughput screening using single-molecule PCR and in vitro expressionYuichi Koga, Katsuya Kato, Hideo Nakano, et al.Biotechnology Progress|April 2, 2005
Enhanced cell-free protein synthesis using a S30 extract from Escherichia coli grown rapidly at 42 degrees C in an amino acid enriched mediumTsuneo Yamane, Yusuke Ikeda, Takehiro Nagasaka, et al.Biotechnology Progress|October 4, 2003
Chimeric gene library construction by a simple and highly versatile method using recombination-dependent exponential amplificationAkinori Ikeuchi, Yasuaki Kawarasaki, Tomoya Shinbata, et al.Protein Engineering|March 28, 2002
Modifying the chain-length selectivity of the lipase from Burkholderia cepacia KWI-56 through in vitro combinatorial mutagenesis in the substrate-binding siteJunhao Yang, Yuichi Koga, Hideo Nakano, et al.Biotechnology Progress|August 24, 2010
A method for reverse interactome analysis: High-resolution mapping of interdomain interaction network in Dam1 complex and its specific disorganization based on the interaction domain expressionAkinori Ikeuchi, Hideo Nakano, Takuma Kamiya, et al.Analytical Biochemistry|March 22, 2002
A method for functional mapping of protein-protein binding domain by preferential amplification of the shortest amplicon using PCRYasuaki Kawarasaki, Yoko Sasaki, Akinori Ikeuchi, et al.Pageof 4